Assessment of Streptomyces sp. and Bacillus sp. Against Phytophthora palmivora, the Causal Agent of Durian Canker Disease through Different Application Methods at The Nursery Stage

  • Nor Dalila Nor Danial
  • Stella Matthews
  • Nurul Fahima Mohamad Amaran
  • Nur Aisyah Anis Abd Kharim

Abstract

Phytophthora palmivora is a natural soil-borne pathogen that infects all parts of the durian tree. It was first reported in 1939 and is still a major problem in durian cultivation. With the increased awareness of the risks of synthetic fungicides usage, the focus of this study is to determine the efficacy of Streptomyces sp. and Bacillus sp. as singular and mixed usage in controlling P. palmivora using drench and spray application methods at the nursery stage. Thus, we aim to identify the relationship of the variables. Microbe treatments consist of; Control, Streptomyces sp. (108 cfu/mL), Bacillus sp. (1010 cfu/mL) and Streptomyces sp. + Bacillus sp. (1010 cfu/mL). The application method was by drenching and spraying method. The experiment consisted of 8 treatments in 4 replications using RCBD Design. Data were computed using SAS to assess descriptive analysis, test of a normality, analysis of variance, multiple comparisons and Pearson correlation at a 95% probability level. The mixture is grouped together with treatment Bacillus sp. in the mean separation using Tukey’s Honestly Significant (HSD). The mixture of Streptomyces sp. and Bacillus sp. was the best in controlling the disease at 19.56 mm lesion growth and 74.35% protection percentage. Here treatment Bacillus sp. has a lesion growth of 28.93 mm and 62.05% protection percentage. All treatments containing microbes had a significant difference compared to control treatments (76.23 mm). As for the application method; the drenching method had a significantly higher plant leaf area of 724.7 cm2 and leaf fresh weight of 18.43 g compared to the spraying method. As for the Pearson correlation coefficient, it is seen the leaf fresh weight (P = <.0001; r = 0.71728) had a significantly strong positive correlation with leaf area. Further studies are needed to verify the effect of Streptomyces sp. and Bacillus sp. at the field level, where these microbes will be subjected to current climate conditions.

Published
30-01-2024
How to Cite
Nor Dalila Nor Danial, Stella Matthews, Nurul Fahima Mohamad Amaran, & Nur Aisyah Anis Abd Kharim. (2024). Assessment of Streptomyces sp. and Bacillus sp. Against Phytophthora palmivora, the Causal Agent of Durian Canker Disease through Different Application Methods at The Nursery Stage. Journal Of Agrobiotechnology, 15(S1), 18-24. https://doi.org/10.37231/jab.2024.15.S1.369