Evaluation of antimalarial and toxicological activities of methanol and water leaves extracts of Piper sarmentosum

Nuk Nur Iman Nik Mat Zin, Munawwarah Omar Khatap, Mohd Dasuki Sul'ain, Nurhidanatasha Abu Bakar

Abstract

The discovery of new antimalarial drugs from medicinal plants is urgently needed due to the development of multidrug-resistant Plasmodium falciparum. Therefore, Piper sarmentosum (kaduk), a commonly used as a herbal medicine to treat malarial symptoms was screened for antimalarial as well as toxicological activities of their methanol and water leaves extracts. The inhibitory concentration (IC50) of P. sarmentosum methanol and water extracts against a chloroquine-sensitive strain (3D7) of P. falciparum was determined by using a malarial SYBR Green I-based fluorescence (MSF) assay. The lethal concentration (LC50) of the plant extracts was evaluated by using a brine shrimp lethality test (BSLT). The presence of heavy metal contents in the crude extract was also detected by using an atomic absorption spectrometry (AAS). Both methanol and water extracts showed an inactive antimalarial activity with an IC50 value of 138.8 ± 0.122 µg/mL and 229.7 ± 0.125 µg/mL, respectively. The water extract was considered non-toxic (LC50 = 2741.7  3.16 ppm), while the methanol extract was toxic (LC50 = 894.94  0.018 ppm). The heavy metals such as plumbum (Pb), zinc (Zn), cadmium (Cd) and chromium (Cr) were identified in the crude extract but they were below the safety limits recommended except for arsenic (As). Further investigations are required to determine the toxicological profiles of P. sarmentosum extracts on mammalian models.

 


Keywords: antimalarial activity; Piper sarmentosum; Plasmodium falciparum; toxicological activity.

 


                                                                                                                                                           

Cite as: Nik Nor Imam Nik Mat Zin, Munawwarah Omar Kathap,Mohd Dasuki Sul’ain et al

(2019). Evaluation of antimalarial and toxicological activities of methanol and water leaves

extracts of Piper sarmentosum. Asian Journal of Medicine & Biomedicine Vol 3:1.

 

 

Date received: February 10th , 2019      Date accepted:  April 17th , 2019

Full Text:

PDF

Refbacks

  • There are currently no refbacks.