Exhaustive Extraction of Compounds from Pomegranate Peel and Flesh Using Solvents of Varying Polarity

  • Idris AM Faculty of Medicine, Universiti Sultan Zainal Abidin
  • Atif AB Faculty of Medicine, Universiti Sultan Zainal Abidin
  • M Adzim KR School of Nutrition and Dietetics, Faculty of Health Sciences, Universiti Sultan Zainal Abidin


Pomegranate fruit is the most studied part of punica granatum shrub. The fruit contains enormous amount of polyphenol compounds in the peel and arils (flesh) which are responsible for its antioxidant activity. The polyphenols present are of varying degree of lipophilicity and thus would require solvents of varying polarity to extract them. In this study, the effects of solvent type and homogenisation on extraction yield were considered. The fruit was first separated into peel and flesh and subsequently, one half of each of the peel and flesh were separately homogenised. Ethanol, ethylacetate and hexane were used to extract the polyphenol content of each of the four samples; non-homogenised peel (NP), non-homogenised flesh (NF), homogenised peel (HP) and homogenised flesh (HF) in decreasing order of polarity using maceration method. The extraction was carried out successively using the residue recovered from previous extraction. Ethanol was used for a second time to complete the extraction process. The total extractive yield from the four samples were 27.19, 26.04, 25.03 and 15.61 for HP, NP, HF and NF respectively. The experiment has demonstrated that maceration process can be used to extract compounds from pomegranate to give a yield similar to more sophisticated method and ethanol is a suitable solvent for extracting hydrophilic compounds from the fruit.


Negi PP, Jayaprakasha GK. Antioxidant and Antibacterial Activities of Punica granatum Peel Extracts. Journal of Food Science. 2006; 68(4): 1473–1477.

Imaida, K., Fukushima, S., Shirui, T., Ohtani, M., Nakanishi, K., Ito, N. Promoting actions of butylated hydroxyl anisole and butylated hydroxy toluene on 2-stage urinary bladder carcinogenesis and ihibition of c-glutamyl transpeptidase-positive foci development in the liver of rats. Carcinogenesis. 1983; 4: 895–899.

Karaaslan M, Vardin H, Varliklioz S, Yilmaz FM. Antiproliferative and antioxidant activities of Turkish pomegranate (Punica granatum L.) accessions. International Journal of Food Science and Technology. 2014; 49: 82–90.

Abdille MH, Singh, RP, Jayaprakasha, GK, Jena, BS. Antioxidant activity of the extracts from Dillenia indica fruits. Food Chemistry. 2005; 90: 891–896.

Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15: 7313-7352.

Chidambara-Murthy KN, Jayaprakasha GK, Singh RP. Studies on antioxidant activity of pomegranate (punica granatum) peel extract using In vivo Models. Journal of Agricultural and Food Chemistry. 2002; 50(1): 4791-4795.

Bravo, L. Polyphenols: Chemistry, dietary sources, metabolism and nutritional significance. Nutrition Reviews. 1998; 56: 317-333.

Qu W, Pan Z, Ma H. extraction modelling and activities of antioxidants from pomegranate marc. Journal of Food Engineering. 2010; 99(1): 16-23.

Cam M, Hisil Y. Pressurised water extraction of polyphenols from pomegranate peels. Food Chemistry. 2010; 123(3): 873-885.

Vaher M, Koel M. Seperation of polyphenolic compounds extracted from plant matrices using capillary electrophoresis. Journal of Chromatography. 2003; 990: 225-230.

Franco D, Sineiro J, Rubilar M,Sanchez M, Jerez M, Pinelo M, Costoya N, Nunez MJ. Polyphenols

Bakels, C. and Jacomet, S., Access to luxury foods in Central Europe during the Roman period: the archaeobotanical evidence, World Archaeology, 34, 542, 2003.

Ward, C., Pomegranates in eastern Mediterranean contexts during the Late Bronze Age, World Arch., 34, 529, 2003.

Riddle, J.M., Oral contraceptives and early-term abortifacients during classical antiquity and the Middle Ages, Past Present, 132, 3, 1991.

Heber, D., Schulman, R. N., & Seeram, N. P. (Eds.). Pomegranates: ancient roots to modern medicine. 2006 CRC press.

Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem 2006; 2(96): 254-260.

Wang Z, Pan Z, Ma H, Atungulu GG. Extracts of phenolics from pomegranate peels. The Open Food Science Journal. 2011; 5: 17-25.

Yasoubi, P., Barzegar, M., Sahari, M. A., & Azizi, M. H. Total phenolic contents and antioxidant activity of pomegranate (Punica granatum L.) peel extracts. Journal of Agricultural Science and Technology.2010 9, 35-42.

Wissam, Zam, Ghada, Bashour, Wassim, Abdelwahed. Effective extraction of polyphenols and anthocyanins from pomegranate’s peels. International Journal of Pharmacy & Pharmaceutical Sciences. 2012; 3(4): 675.

How to Cite
AM, I., AB, A., & KR, M. A. (2017). Exhaustive Extraction of Compounds from Pomegranate Peel and Flesh Using Solvents of Varying Polarity. Asian Journal of Medicine and Biomedicine, 1(1), 10-13. Retrieved from https://journal.unisza.edu.my/ajmb/index.php/ajmb/article/view/21