Electrotransformation on Selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum

  • WAI KEONG LOKE UNIVERSITI PUTRA MALAYSIA
  • Halimi Mohd Saud

Abstract

Electrotransformation was successfully transferred a 21.6 kb cosmid vector pLAFR1 into the selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum with maximum efficiency about 107 transformants/µg at electric field strength of 10kV/cm for shorter pulse length range between 4.1 to 4.4 milliseconds and about 108 transformants/µg at electric field strength of 5kV/cm for a longer pulse length range between 8.5 to 8.7 milliseconds. Longer incubation period will increase the transformation efficiency but continuing increasing the period after 20 hours will decrease the transformation efficiency.

 

Published
26-05-2020
How to Cite
LOKE, W. K., & Mohd Saud, H. (2020). Electrotransformation on Selected Plant Growth-Promoting Rhizobacteria (PGPR) and Chromobacterium violaceum. Journal Of Agrobiotechnology, 11(1), 63-69. https://doi.org/10.37231/jab.2020.11.1.214
Section
Articles