Production of Phytate-Degrading Enzyme from Malaysian Soil Bacteria Using Rice Bran Containing Media

  • Anis Shobirin Meor Hussin Universiti Putra Malaysia
  • Abd-ElAziem Farouk University of Taif
  • A.M Ali Universiti Darul Iman Malaysia
  • R. Greiner Max Rubner-Institute

Abstract

The aims of the study were to observe the effects of different concentration of rice bran in differentmedia on phytase synthesis and to optimize the temperature and pH of the media for phytaseproduction by those bacterial strains. Three bacterial strain isolates obtained from the soil of Malaysianmaize plantation were used to produce phytase. In this study, the effects of different rice branconcentration, incubation temperature and initial pH-values of the media on phytase production wereevaluated. Incorporation of 7.5% rice bran has the inducible effect on all the bacterial tested. In respectto phytase production, the best cultivation media and cultivation time for Bacillus cereus ASUIA260 wasPFE with 7.5% rice bran after 3 days, whilst for Pantoea stewartii ASUIA271 and Enterobacter sakazakii ASUIA279, it was LB with 7.5% rice bran after 3 days and 5 days, respectively. The arrangement ofthose isolates according to their ability to produce phytases were E. sakazakii ASUIA279 > P. stewartii ASUIA271 > B. cereus ASUIA260. Production of phytase by those bacteria was triggered by the highcontent of organic phytate in the rice bran. Optimum temperature for phytase production of B. cereus ASUIA260 was 41 C compared to P. stewartii ASUIA271 and E. sakazakii ASUIA279 with 33 C and37 C, respectively. Optimum initial pH for phytase production of B. cereus ASUIA 260 was pH 7.2,while P. stewartii ASUIA271 and E. sakazakii ASUIA 279 were both at pH 6.0.

Author Biographies

Anis Shobirin Meor Hussin, Universiti Putra Malaysia
Department of Food Technology, Faculty of Science and Technology
Abd-ElAziem Farouk, University of Taif
Faculty of Science
A.M Ali, Universiti Darul Iman Malaysia
Faculty of Agriculture and Biotechnology
R. Greiner, Max Rubner-Institute
Department of Food and BioProcess Engineering
Published
16-03-2011
How to Cite
Meor Hussin, A. S., Farouk, A.-E., Ali, A., & Greiner, R. (2011). Production of Phytate-Degrading Enzyme from Malaysian Soil Bacteria Using Rice Bran Containing Media. Journal Of Agrobiotechnology, 1, 17-28. Retrieved from https://journal.unisza.edu.my/agrobiotechnology/index.php/agrobiotechnology/article/view/3
Section
Articles